A Comparison of Genome-Wide DNA Methylation Patterns between Different Vascular Tissues from Patients with Coronary Heart Disease

Title: A Comparison of Genome-Wide DNA Methylation Patterns between Different Vascular Tissues from Patients with Coronary Heart Disease
Authors: Nazarenko, MS
Markov, AV
Lebedev, IN
Freidin, MB
Sleptcov, AA
Koroleva, IA
Frolov, AV
Popov, VA
Barbarash, OL
Puzyrev, VP
Item Type: Journal Article
Abstract: Epigenetic mechanisms of gene regulation in context of cardiovascular diseases are of considerable interest. So far, our current knowledge of the DNA methylation profiles for atherosclerosis affected and healthy human vascular tissues is still limited. Using the Illumina Infinium Human Methylation27 BeadChip, we performed a genome-wide analysis of DNA methylation in right coronary artery in the area of advanced atherosclerotic plaques, atherosclerotic-resistant internal mammary arteries, and great saphenous veins obtained from same patients with coronary heart disease. The resulting DNA methylation patterns were markedly different between all the vascular tissues. The genes hypomethylated in athero-prone arteries to compare with atherosclerotic-resistant arteries were predominately involved in regulation of inflammation and immune processes, as well as development. The great saphenous veins exhibited an increase of the DNA methylation age in comparison to the internal mammary arteries. Gene ontology analysis for genes harboring hypermethylated CpG-sites in veins revealed the enrichment for biological processes associated with the development. Four CpG-sites located within the MIR10B gene sequence and about 1 kb upstream of the HOXD4 gene were also confirmed as hypomethylated in the independent dataset of the right coronary arteries in the area of advanced atherosclerotic plaques in comparison with the other vascular tissues. The DNA methylation differences observed in vascular tissues of patients with coronary heart disease can provide new insights into the mechanisms underlying the development of pathology and explanation for the difference in graft patency after coronary artery bypass grafting surgery.
Issue Date: 9-Apr-2015
Date of Acceptance: 11-Feb-2015
ISSN: 1932-6203
Publisher: Public Library of Science
Journal / Book Title: PLOS One
Volume: 10
Issue: 4
Copyright Statement: © 2015 Nazarenko et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Aged
Coronary Disease
Coronary Vessels
CpG Islands
DNA Methylation
Epigenesis, Genetic
Genome-Wide Association Study
Homeodomain Proteins
Mammary Arteries
Middle Aged
Molecular Sequence Annotation
Organ Specificity
Plaque, Atherosclerotic
Saphenous Vein
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Article Number: e0122601
Appears in Collections:National Heart and Lung Institute
Airway Disease
Faculty of Medicine

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