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Physical proximity of sister chromatids promotes top2-dependent intertwining

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Title: Physical proximity of sister chromatids promotes top2-dependent intertwining
Authors: Sen, N
Leonard, J
Torres, R
Garcia-Luis, J
Palou-Marin, G
Aragon Alcaide, L
Item Type: Journal Article
Abstract: Sister chromatid intertwines (SCIs), or catenanes, are topological links between replicated chromatids that interfere with chromosome segregation. The forma- tion of SCIs is thought to be a consequence of fork swiveling during DNA replication, and their removal is thought to occur because of the intrinsic feature of type II topoisomerases (Top2) to simplify DNA topology. Here, we report that SCIs are also formed independently of DNA replication during G 2 /M by Top2-dependent concatenation of cohesed chroma- tids due to their physical proximity. We demonstrate that, in contrast to G 2 /M, Top2 removes SCIs from co- hesedchromatidsattheanaphaseonset.Importantly, SCI removal in anaphase requires condensin and co- incides with the hyperactivation of condensin DNA supercoiling activity. This is consistent with the long- standing proposal that condensin provides a bias in Top2functiontowarddecatenation.Acomprehensive model for the formation and resolution of toxic SCI entanglements on eukaryotic genomes is proposed.
Issue Date: 6-Oct-2016
Date of Acceptance: 6-Sep-2016
URI: http://hdl.handle.net/10044/1/40855
DOI: 10.1016/j.molcel.2016.09.007
ISSN: 1097-4164
Publisher: Elsevier (Cell Press)
Start Page: 134
End Page: 147
Journal / Book Title: Molecular Cell
Volume: 64
Issue: 1
Copyright Statement: 2016 Crown Copyright © 2016 Published by Elsevier Inc This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 100955/Z/13/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
MITOTIC CHROMOSOME CONDENSATION
TOPOISOMERASE-II
SACCHAROMYCES-CEREVISIAE
DNA TOPOISOMERASES
IN-VITRO
COHESION
DECATENATION
TOPOLOGY
PROTEIN
MITOSIS
DNA replication
SCI
Top2 (Topoisomerase 2)
catenation
chromosome segregation
cohesin
condensin
Adenosine Triphosphatases
Anaphase
Chromatids
Chromosome Segregation
Chromosomes, Fungal
DNA Replication
DNA Topoisomerases, Type II
DNA, Fungal
DNA-Binding Proteins
G2 Phase Cell Cycle Checkpoints
Gene Expression
Multiprotein Complexes
Saccharomyces cerevisiae
Chromosomes, Fungal
Chromatids
Saccharomyces cerevisiae
Multiprotein Complexes
DNA Topoisomerases, Type II
DNA-Binding Proteins
DNA, Fungal
Anaphase
Chromosome Segregation
DNA Replication
Gene Expression
Adenosine Triphosphatases
G2 Phase Cell Cycle Checkpoints
Developmental Biology
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2016-10-06
Appears in Collections:Institute of Clinical Sciences
Faculty of Medicine