A Bioreactor to Apply Multimodal Physical Stimuli to Cultured Cells

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Title: A Bioreactor to Apply Multimodal Physical Stimuli to Cultured Cells
Authors: Edelmann, JC
Jones, L
Peyronnet, R
Lu, L
Kohl, P
Ravens, U
Item Type: Journal Article
Abstract: Cells residing in the cardiac niche are constantly experiencing physical stimuli, including electrical pulses and cyclic mechanical stretch. These physical signals are known to influence a variety of cell functions, including the secretion of growth factors and extracellular matrix proteins by cardiac fibroblasts, calcium handling and contractility in cardiomyocytes, or stretch-activated ion channels in muscle and non-muscle cells of the cardiovascular system. Recent progress in cardiac tissue engineering suggests that controlled physical stimulation can lead to functional improvements in multicellular cardiac tissue constructs. To study these effects, aspects of the physical environment of the myocardium have to be mimicked in vitro. Applying continuous live imaging, this protocol demonstrates how a specifically designed bioreactor system allows controlled exposure of cultured cells to cyclic stretch, rhythmic electrical stimulation, and controlled fluid perfusion, at user-defined temperatures.
Issue Date: 1-Apr-2016
Date of Acceptance: 1-Jan-2016
URI: http://hdl.handle.net/10044/1/39082
DOI: https://dx.doi.org/10.1007/7651_2016_336
ISSN: 1940-6029
Publisher: Springer
Start Page: 21
End Page: 33
Journal / Book Title: Methods in Molecular Biology
Volume: 1502
Copyright Statement: The final publication is available at Springer via http://dx.doi.org/10.1007/7651_2016_336
Keywords: Bioreactor
Cell mechanics
Cyclic stretch
Electrical stimulation
Excitable cells
Developmental Biology
0601 Biochemistry And Cell Biology
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine

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