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Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

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Title: Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain
Authors: Florea, M
Hagemann, H
Santosa, G
Abbott, J
Micklem, CN
Spencer-Milnes, X
De Arroyo Garcia, L
Paschou, D
Lazenbatt, C
Kong, D
Chughtai, H
Jensen, K
Freemont, P
Kitney, RI
Reeve, B
Ellis, T
Item Type: Journal Article
Abstract: Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae. Its high strength, purity and biocompatibility make it of great interest to materials science, however precise control of its biosynthesis has remained a challenge for biotechnology. Here we isolate a new strain of Komagataeibacter rhaeticus (Komagataeibacter rhaeticus iGEM) that can produce cellulose at high yields, grow in low nitrogen conditions, and is highly resistant to toxic chemicals. We achieve external control over its bacterial cellulose production through development of a modular genetic toolkit that enables rational reprogramming of the cell. To further its use as an organism for biotechnology, we sequenced its genome and demonstrate genetic circuits that enable functionalization and patterning of heterologous gene expression within the cellulose matrix. This work lays the foundations for using genetic engineering to produce cellulose-based materials, with numerous applications in basic science, materials engineering and biotechnology.
Issue Date: 14-Jun-2016
Date of Acceptance: 29-Apr-2016
URI: http://hdl.handle.net/10044/1/33165
DOI: 10.1073/pnas.1522985113
ISSN: 0027-8424
Publisher: National Academy of Sciences
Start Page: E3431
End Page: E3440
Journal / Book Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 113
Issue: 24
Copyright Statement: Freely available online through the PNAS open access option
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/M002306/1
EP/J02175X/1
EP/L011573/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
synthetic biology
bacterial cellulose
genetic engineering
biomaterials
genomics
ACETOBACTER-XYLINUM
SYNTHETIC BIOLOGY
ESCHERICHIA-COLI
GENOME SEQUENCE
BINDING DOMAIN
GLUCONACETOBACTER-XYLINUS
SUGAR-CANE
DIAZOTROPHICUS
EXPRESSION
PROTEIN
bacterial cellulose
biomaterials
genetic engineering
genomics
synthetic biology
Cellulose
Gram-Positive Asporogenous Rods
Metabolic Engineering
Gram-Positive Asporogenous Rods
Cellulose
Metabolic Engineering
Publication Status: Published
Open Access location: http://pnas.org/content/early/2016/05/26/1522985113.full
Online Publication Date: 2016-05-31
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Bioengineering
Department of Infectious Diseases
Faculty of Medicine
Faculty of Natural Sciences
Faculty of Engineering