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Aptamer-conjugated, fluorescent gold nanorods as potential cancer theradiagnostic agents

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Title: Aptamer-conjugated, fluorescent gold nanorods as potential cancer theradiagnostic agents
Authors: Gallina, ME
Zhou, Y
Johnson, CJ
Harris-Birtill, D
Singh, M
Zhao, H
Ma, D
Cass, T
Elson, DS
Item Type: Journal Article
Abstract: GNRs are emerging as a new class of probes for theradiagnostic applications thanks to their unique optical properties. However, the achievement of proper nanoconstructs requires the synthesis of highly pure GNRs with well-defined aspect ratio (AR), in addition to extensive surface chemistry modification to provide them with active targeting and, possibly, multifunctionality. In this work, we refined the method of the seed mediated growth and developed a robust procedure for the fabrication of GNRs with specific AR. We also revealed and characterized unexplored aging phenomena that follow the synthesis and consistently alter GNRs' final AR. Such advances appreciably improved the feasibility of GNRs fabrication and offered useful insights on the growth mechanism. We next produced fluorescent, biocompatible, aptamer-conjugated GNRs by performing ligand exchange followed by bioconjugation to anti-cancer oligonucleotide AS1411. In vitro studies showed that our nanoconstructs selectively target cancer cells while showing negligible cytotoxicity. As a result, our aptamer-conjugated GNRs constitute ideal cancer-selective multifunctional probes and promising candidates as photothermal therapy agents.
Issue Date: 1-Feb-2016
Date of Acceptance: 28-Sep-2015
URI: http://hdl.handle.net/10044/1/32573
DOI: 10.1016/j.msec.2015.09.101
ISSN: 0928-4931
Publisher: Elsevier
Start Page: 324
End Page: 332
Journal / Book Title: Materials Science and Engineering C-Biomimetic and Supramolecular Systems
Volume: 59
Issue: 1
Copyright Statement: © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Sponsor/Funder: Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: 242991
EP/K039946/1
Keywords: Science & Technology
Technology
Materials Science, Biomaterials
Materials Science
Gold nanorods
Aptamer
Targeted cancer labelling
Fluorescence microscopy
Photothermal therapy
PHOTOTHERMAL THERAPY
GROWTH-MECHANISM
IN-VITRO
NANOPARTICLES
STABILITY
CHEMOTHERAPY
ABSORPTION
SCATTERING
DRIVEN
CELLS
Aptamer
Fluorescence microscopy
Gold nanorods
Photothermal therapy
Targeted cancer labelling
Aptamers, Nucleotide
Fluorescent Dyes
Gold
HeLa Cells
Humans
Nanotubes
Neoplasms
Phototherapy
Hela Cells
Humans
Neoplasms
Gold
Fluorescent Dyes
Phototherapy
Nanotubes
Aptamers, Nucleotide
Biomedical Engineering
0903 Biomedical Engineering
0912 Materials Engineering
Publication Status: Published
Online Publication Date: 2015-10-03
Appears in Collections:Department of Surgery and Cancer
Chemistry
Biological and Biophysical Chemistry
Faculty of Medicine
Faculty of Natural Sciences