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Single molecule analysis of functionally asymmetric G protein-coupled receptor (GPCR) oligomers reveals diverse spatial and structural assemblies
| File | Description | Size | Format | |
|---|---|---|---|---|
 J. Biol. Chem.-2015-Jonas-3875-92(1).pdf | Published version | 4.33 MB | Adobe PDF | View/Open |
| Title: | Single molecule analysis of functionally asymmetric G protein-coupled receptor (GPCR) oligomers reveals diverse spatial and structural assemblies |
| Authors: | Jonas, KC Fanelli, F Huhtaniemi, IT Hanyaloglu, AC |
| Item Type: | Journal Article |
| Abstract: | Background: GPCRs form complex oligomers whose role in signaling is poorly understood. Results: Super-resolution imaging of functionally asymmetric oligomers reveals diverse functional and structural organizations and the ability to alter signal responses. Conclusion: GPCR oligomers may fine-tune receptor signaling by altering the functional role of individual protomers. Significance: Distinct oligomers could be exploited pharmacologically to improve efficacy, selectivity, and/or specificity. |
| Issue Date: | 13-Feb-2015 |
| Date of Acceptance: | 5-Dec-2014 |
| URI: | http://hdl.handle.net/10044/1/31311 |
| DOI: | 10.1074/jbc.M114.622498 |
| ISSN: | 1083-351X |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Start Page: | 3875 |
| End Page: | 3892 |
| Journal / Book Title: | Journal of Biological Chemistry |
| Volume: | 290 |
| Issue: | 7 |
| Copyright Statement: | © 2014 The American Society for Biochemistry and Molecular Biology, Inc. Available under Creative Commons Attribution Unported License. |
| Sponsor/Funder: | Biotechnology and Biological Sciences Research Council (BBSRC) Wellcome Trust |
| Funder's Grant Number: | BB/I008004/1 085099/Z/08/Z |
| Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology LUTEINIZING-HORMONE RECEPTORS FOLLICLE-STIMULATING-HORMONE PHOTOACTIVATED LOCALIZATION MICROSCOPY BETA(2) ADRENERGIC-RECEPTOR RESONANCE ENERGY-TRANSFER CRYSTAL-STRUCTURE BETA-2-ADRENERGIC RECEPTOR LH RECEPTOR CELLS HOMODIMERIZATION Bioluminescence Resonance Energy Transfer (BRET) Cell Signaling Dimer G Protein G protein-Coupled Receptor (GPCR) Oligomer Structural Biology Super-resolution Imaging Fluorescent Dyes HEK293 Cells Humans Image Processing, Computer-Assisted Models, Molecular Protein Binding Protein Conformation Protein Multimerization Receptors, LH Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Humans Receptors, LH Fluorescent Dyes Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Protein Conformation Protein Binding Models, Molecular Image Processing, Computer-Assisted Protein Multimerization HEK293 Cells 03 Chemical Sciences 06 Biological Sciences 11 Medical and Health Sciences Biochemistry & Molecular Biology |
| Publication Status: | Published |
| Online Publication Date: | 2015-02-13 |
| Appears in Collections: | Department of Metabolism, Digestion and Reproduction Faculty of Medicine |