Instrumentation to Study Myofibril Mechanics from Static to Artificial Simulations of Cardiac Cycle

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Title: Instrumentation to Study Myofibril Mechanics from Static to Artificial Simulations of Cardiac Cycle
Authors: Vikhorev, P
Marston, S
Ferenczi, M
Item Type: Journal Article
Abstract: © 2016 The Authors.Many causes of heart muscle diseases and skeletal muscle diseases are inherited and caused by mutations in genes of sarcomere proteins which play either a structural or contractile role in the muscle cell. Tissue samples from human hearts with mutations can be obtained but often samples are only a few milligrams and it is necessary to freeze them for storage and transportation. Myofibrils are the fundamental contractile components of the muscle cell and retain all structural elements and contractile proteins performing in contractile event; moreover viable myofibrils can be obtained from frozen tissue. We are describing a versatile technique for measuring the contractility and its Ca2+ regulation in single myofibrils. The control of myofibril length, incubation medium and data acquisition is carried out using a digital acquisition board via computer software. Using computer control it is possible not only to measure contractile and mechanical parameters but also simulate complex protocols such as a cardiac cycle to vary length and medium independently.This single myofibril force assay is well suited for physiological measurements. The system can be adapted to measure tension amplitude, rates of contraction and relaxation, Ca2+ dependence of these parameters in dose-response measurements, length-dependent activation, stretch response, myofibril elasticity and response to simulated cardiac cycle length changes. Our approach provides an all-round quantitative way to measure myofibrils performance and to observe the effect of mutations or posttranslational modifications. The technique has been demonstrated by the study of contraction in heart with hypertrophic or dilated cardiomyopathy mutations in sarcomere proteins.
Issue Date: 2-Mar-2016
Date of Acceptance: 28-Feb-2016
URI: http://hdl.handle.net/10044/1/29910
DOI: https://dx.doi.org/10.1016/j.mex.2016.02.006
ISSN: 2215-0161
Publisher: Elsevier
Start Page: 156
End Page: 170
Journal / Book Title: MethodsX
Volume: 3
Copyright Statement: © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Sponsor/Funder: British Heart Foundation
Funder's Grant Number: RG/11/20/29266
Publication Status: Published
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine



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