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easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy

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Kwakwa_et_al-2016-Journal_of_Biophotonics.pdfPublished version372.59 kBAdobe PDFView/Open
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Title: easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy
Authors: Kwakwa, K
Savell, A
Davies, T
Munro, I
Parrinello, S
Purbhoo, MA
Dunsby, C
Neil, MAA
French, PMW
Item Type: Journal Article
Abstract: TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.
Issue Date: 11-Mar-2016
Date of Acceptance: 31-Jan-2016
URI: http://hdl.handle.net/10044/1/29174
DOI: http://dx.doi.org/10.1002/jbio.201500324
ISSN: 1864-0648
Publisher: Wiley-VCH Verlag
Start Page: 948
End Page: 957
Journal / Book Title: Journal of Biophotonics
Volume: 9
Issue: 9
Copyright Statement: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Medical Research Council (MRC)
Medical Research Council
Funder's Grant Number: 093465/Z/10/Z
Keywords: Optoelectronics & Photonics
Publication Status: Published
Appears in Collections:Physics
Department of Surgery and Cancer
Faculty of Natural Sciences