27
IRUS Total
Downloads
  Altmetric

B'-protein phosphatase 2A is a functional binding partner of delta-retroviral integrase

File Description SizeFormat 
Maertens GN_accepted.pdfAccepted version1.44 MBAdobe PDFView/Open
Nucl. Acids Res.-2016-Maertens-364-76.pdfPublished version6.79 MBAdobe PDFView/Open
Title: B'-protein phosphatase 2A is a functional binding partner of delta-retroviral integrase
Authors: Maertens, GNE
Item Type: Journal Article
Abstract: To establish infection, a retrovirus must insert a DNA copy of its RNA genome into host chromatin. This reaction is catalysed by the virally encoded enzyme integrase (IN) and is facilitated by viral genus-specific host factors. Herein, cellular serine/threonine protein phosphatase 2A (PP2A) is identified as a functional IN binding partner exclusive to δ-retroviruses, including human T cell lymphotropic virus type 1 and 2 (HTLV-1 and HTLV-2) and bovine leukaemia virus (BLV). PP2A is a heterotrimer composed of a scaffold, catalytic and one of any of four families of regulatory subunits, and the interaction is specific to the B′ family of the regulatory subunits. B′-PP2A and HTLV-1 IN display nuclear co-localization, and the B′ subunit stimulates concerted strand transfer activity of δ-retroviral INs in vitro. The protein–protein interaction interface maps to a patch of highly conserved residues on B′, which when mutated render B′ incapable of binding to and stimulating HTLV-1 and -2 IN strand transfer activity.
Issue Date: 8-Jan-2016
Date of Acceptance: 19-Nov-2015
URI: http://hdl.handle.net/10044/1/27828
DOI: 10.1093/nar/gkv1347
ISSN: 1362-4962
Publisher: Oxford University Press (OUP)
Start Page: 364
End Page: 376
Journal / Book Title: Nucleic Acids Research
Volume: 44
Issue: 1
Copyright Statement: © The Author 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Wellcome Trust
Funder's Grant Number: 107005/Z/15/Z
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
LEUKEMIA-VIRUS INTEGRATION
TRANSCRIPTION START SITES
REGULATORY SUBUNITS
B56-CONTAINING PP2AS
HIV-1 INTEGRATION
TYPE-1 INFECTION
FAMILY
PHOSPHORYLATION
B56-GAMMA
LEDGF/P75
Amino Acid Sequence
Animals
Carrier Proteins
Cattle
Cell Line
Deltaretrovirus
Enzyme Activation
Human T-lymphotropic virus 1
Humans
Integrases
Leukemia Virus, Bovine
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Protein Phosphatase 2
Protein Subunits
Sequence Alignment
Virus Integration
Cell Line
Animals
Cattle
Humans
Deltaretrovirus
Leukemia Virus, Bovine
Human T-lymphotropic virus 1
Integrases
Carrier Proteins
Protein Subunits
Sequence Alignment
Protein Interaction Mapping
Virus Integration
Enzyme Activation
Amino Acid Sequence
Protein Conformation
Protein Binding
Models, Molecular
Molecular Sequence Data
Protein Phosphatase 2
Protein Interaction Domains and Motifs
05 Environmental Sciences
06 Biological Sciences
08 Information and Computing Sciences
Developmental Biology
Publication Status: Published
Online Publication Date: 2015-12-10
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine