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Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.

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Nat. Mater Nanoneedles main text_JOM_EDR_CC_ET.docxAccepted version85.25 kBMicrosoft WordView/Open
Nat. Mater Nanoneedles Supplementary Material_2.docxSupporting information12.63 MBMicrosoft WordView/Open
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Title: Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.
Authors: Chiappini, C
De Rosa, E
Martinez, JO
Liu, X
Steele, J
Stevens, MM
Tasciotti, E
Item Type: Journal Article
Abstract: The controlled delivery of nucleic acids to selected tissues remains an inefficient process mired by low transfection efficacy, poor scalability because of varying efficiency with cell type and location, and questionable safety as a result of toxicity issues arising from the typical materials and procedures employed. High efficiency and minimal toxicity in vitro has been shown for intracellular delivery of nuclei acids by using nanoneedles, yet extending these characteristics to in vivo delivery has been difficult, as current interfacing strategies rely on complex equipment or active cell internalization through prolonged interfacing. Here, we show that a tunable array of biodegradable nanoneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.
Issue Date: 1-May-2015
Date of Acceptance: 11-Feb-2015
URI: http://hdl.handle.net/10044/1/21906
DOI: 10.1038/nmat4249
ISSN: 1476-1122
Publisher: Nature Research
Start Page: 532
End Page: 539
Journal / Book Title: Nature Materials
Volume: 14
Issue: 5
Copyright Statement: © 2015, Rights Managed by Nature Publishing Group
Sponsor/Funder: Commission of the European Communities
Wellcome Trust
Funder's Grant Number: ERC-2013-CoG-616417
098411/Z/12/Z
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Physical
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Materials Science
Physics
POROUS SILICON
GENE-TRANSFER
GROWTH-FACTORS
LIVING CELLS
DRUG-DELIVERY
ELECTROPORATION
PLATFORM
MICROVESICLES
RNA
THERAPEUTICS
Animals
Humans
Mice
Muscle, Skeletal
Nanostructures
Needles
Neovascularization, Physiologic
Plasmids
Silicon
Transfection
Vascular Endothelial Growth Factor A
Muscle, Skeletal
Animals
Humans
Mice
Silicon
Vascular Endothelial Growth Factor A
Transfection
Needles
Neovascularization, Physiologic
Plasmids
Nanostructures
Nanoscience & Nanotechnology
Publication Status: Published
Online Publication Date: 2015-03-30
Appears in Collections:Materials
Faculty of Natural Sciences
Faculty of Engineering