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3D imaging of cement-based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioning
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3D imaging of cement based materials at submicron resolution - accepted.pdf | Accepted version | 2.56 MB | Adobe PDF | View/Open |
Title: | 3D imaging of cement-based materials at submicron resolution by combining laser scanning confocal microscopy with serial sectioning |
Authors: | Yio, MHN Mac, MJ Wong, HS Buenfeld, NR |
Item Type: | Journal Article |
Abstract: | In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z-stacks, which are then aligned and stitched based on phase correlation. The method can be extended in the XY plane to further increase the overall image volume. Resolution of the reconstructed image volume does not degrade with increase in sample size. We have used the method to image cementitious materials, hardened cement paste and concrete and the results obtained show that the method is reliable. Possible applications of the method such as three-dimensional characterization of the pores and microcracks in hardened concrete, three-dimensional particle shape characterization of cementitious materials and three-dimensional characterization of other porous materials such as rocks and bioceramics are discussed. |
Issue Date: | 1-May-2015 |
Date of Acceptance: | 30-Dec-2014 |
URI: | http://hdl.handle.net/10044/1/21543 |
DOI: | 10.1111/jmi.12228 |
ISSN: | 0022-2720 |
Publisher: | Wiley |
Start Page: | 151 |
End Page: | 169 |
Journal / Book Title: | Journal of Microscopy |
Volume: | 258 |
Issue: | 2 |
Copyright Statement: | © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society. This is the peer reviewed version of the following article: Journal of Microscopy, Volume 258, Issue 2, pages 151–169, May 2015, which has been published in final form at https://dx.doi.org/10.1111/jmi.12228. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving. |
Keywords: | Science & Technology Technology Microscopy Cement-based materials laser scanning confocal microscopy microcrack microstructure 3D imaging pore structure serial sectioning X-RAY MICROTOMOGRAPHY FIB-NANOTOMOGRAPHY PARTICULATE SYSTEMS PORE STRUCTURE RECONSTRUCTION SHAPE MICROSTRUCTURES ROUGHNESS POROSITY PASTE 3D imaging Cement-based materials laser scanning confocal microscopy microcrack microstructure pore structure serial sectioning 0204 Condensed Matter Physics 0601 Biochemistry and Cell Biology 0912 Materials Engineering Microscopy |
Publication Status: | Published |
Online Publication Date: | 2015-02-04 |
Appears in Collections: | Civil and Environmental Engineering Faculty of Engineering |