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Exome sequencing followed by large-scale genotyping suggests a limited role for moderately rare risk factors of strong effect in schizophrenia.

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Title: Exome sequencing followed by large-scale genotyping suggests a limited role for moderately rare risk factors of strong effect in schizophrenia.
Authors: Need, AC
McEvoy, JP
Gennarelli, M
Heinzen, EL
Ge, D
Maia, JM
Shianna, KV
He, M
Cirulli, ET
Gumbs, CE
Zhao, Q
Campbell, CR
Hong, L
Rosenquist, P
Putkonen, A
Hallikainen, T
Repo-Tiihonen, E
Tiihonen, J
Levy, DL
Meltzer, HY
Goldstein, DB
Item Type: Journal Article
Abstract: Schizophrenia is a severe psychiatric disorder with strong heritability and marked heterogeneity in symptoms, course, and treatment response. There is strong interest in identifying genetic risk factors that can help to elucidate the pathophysiology and that might result in the development of improved treatments. Linkage and genome-wide association studies (GWASs) suggest that the genetic basis of schizophrenia is heterogeneous. However, it remains unclear whether the underlying genetic variants are mostly moderately rare and can be identified by the genotyping of variants observed in sequenced cases in large follow-up cohorts or whether they will typically be much rarer and therefore more effectively identified by gene-based methods that seek to combine candidate variants. Here, we consider 166 persons who have schizophrenia or schizoaffective disorder and who have had either their genomes or their exomes sequenced to high coverage. From these data, we selected 5,155 variants that were further evaluated in an independent cohort of 2,617 cases and 1,800 controls. No single variant showed a study-wide significant association in the initial or follow-up cohorts. However, we identified a number of case-specific variants, some of which might be real risk factors for schizophrenia, and these can be readily interrogated in other data sets. Our results indicate that schizophrenia risk is unlikely to be predominantly influenced by variants just outside the range detectable by GWASs. Rather, multiple rarer genetic variants must contribute substantially to the predisposition to schizophrenia, suggesting that both very large sample sizes and gene-based association tests will be required for securely identifying genetic risk factors. © 2012 The American Society of Human Genetics.
Issue Date: 10-Aug-2012
URI: http://hdl.handle.net/10044/1/20036
DOI: http://dx.doi.org/10.1016/j.ajhg.2012.06.018
Start Page: 303
End Page: 312
Journal / Book Title: Am J Hum Genet
Volume: 91
Issue: 2
Copyright Statement: Copyright © 2012 Elsevier Ltd. All rights reserved. NOTICE: this is the author’s version of a work that was accepted for publication in American Journal of Human Genetics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in American Journal of Human Genetics, 91(2), 2012. DOI:10.1016/j.ajhg.2012.06.018
Conference Place: United States
Appears in Collections:Department of Medicine (up to 2019)