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Porous nucleating agents for protein crystallization
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![]() | Accepted version | 923.26 kB | Adobe PDF | View/Open |
Title: | Porous nucleating agents for protein crystallization |
Authors: | Khurshid, S Saridakis, E Govada, L Chayen, NE |
Item Type: | Journal Article |
Abstract: | Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months. |
Issue Date: | 1-Jul-2014 |
Date of Acceptance: | 1-Jun-2014 |
URI: | http://hdl.handle.net/10044/1/19210 |
DOI: | 10.1038/nprot.2014.109 |
ISSN: | 1750-2799 |
Publisher: | Nature Research |
Start Page: | 1621 |
End Page: | 1633 |
Journal / Book Title: | Nature Protocols |
Volume: | 9 |
Issue: | 7 |
Copyright Statement: | © 2014 Nature Publishing Group |
Sponsor/Funder: | Engineering & Physical Science Research Council (EPSRC) Engineering & Physical Science Research Council (E Engineering & Physical Science Research Council (EPSRC) |
Funder's Grant Number: | EP/G027005/1 EP/K503733/1 EP/G014736/1 |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology MACROMOLECULAR CRYSTAL-GROWTH HETEROGENEOUS NUCLEATION MEMBRANE-PROTEINS VAPOR DIFFUSION PHASE-DIAGRAM SURFACES OPTIMIZATION PURIFICATION TEMPLATE STRATEGY Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology MACROMOLECULAR CRYSTAL-GROWTH HETEROGENEOUS NUCLEATION MEMBRANE-PROTEINS VAPOR DIFFUSION PHASE-DIAGRAM SURFACES OPTIMIZATION PURIFICATION TEMPLATE STRATEGY Ceramics Crystallization Filtration Phase Transition Porosity Protein Stability Proteins X-Ray Diffraction Proteins Ceramics Crystallization X-Ray Diffraction Filtration Phase Transition Porosity Protein Stability Bioinformatics 03 Chemical Sciences 06 Biological Sciences 11 Medical and Health Sciences |
Publication Status: | Published |
Online Publication Date: | 2014-06-12 |
Appears in Collections: | Department of Metabolism, Digestion and Reproduction Faculty of Medicine Faculty of Natural Sciences |