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Porous nucleating agents for protein crystallization

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Title: Porous nucleating agents for protein crystallization
Authors: Khurshid, S
Saridakis, E
Govada, L
Chayen, NE
Item Type: Journal Article
Abstract: Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.
Issue Date: 1-Jul-2014
Date of Acceptance: 1-Jun-2014
URI: http://hdl.handle.net/10044/1/19210
DOI: 10.1038/nprot.2014.109
ISSN: 1750-2799
Publisher: Nature Research
Start Page: 1621
End Page: 1633
Journal / Book Title: Nature Protocols
Volume: 9
Issue: 7
Copyright Statement: © 2014 Nature Publishing Group
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/G027005/1
EP/K503733/1
EP/G014736/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
MACROMOLECULAR CRYSTAL-GROWTH
HETEROGENEOUS NUCLEATION
MEMBRANE-PROTEINS
VAPOR DIFFUSION
PHASE-DIAGRAM
SURFACES
OPTIMIZATION
PURIFICATION
TEMPLATE
STRATEGY
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
MACROMOLECULAR CRYSTAL-GROWTH
HETEROGENEOUS NUCLEATION
MEMBRANE-PROTEINS
VAPOR DIFFUSION
PHASE-DIAGRAM
SURFACES
OPTIMIZATION
PURIFICATION
TEMPLATE
STRATEGY
Ceramics
Crystallization
Filtration
Phase Transition
Porosity
Protein Stability
Proteins
X-Ray Diffraction
Proteins
Ceramics
Crystallization
X-Ray Diffraction
Filtration
Phase Transition
Porosity
Protein Stability
Bioinformatics
03 Chemical Sciences
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2014-06-12
Appears in Collections:Department of Metabolism, Digestion and Reproduction
Faculty of Medicine
Faculty of Natural Sciences