Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens

Title: Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens
Authors: Willems, E
Gloerich, J
Suppers, A
Van der Flier, M
Van den Heuvel, LP
Van de Kar, N
Philipsen, RHLA
Van Dael, M
Kaforou, M
Wright, VJ
Herberg, JA
Torres, FM
Levin, M
De Groot, R
Van Gool, AJ
Lefeber, DJ
Wessels, HJCT
De Jonge, MI
PERFORM consortium
Item Type: Journal Article
Abstract: Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttranslational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic proteome-wide glycosylation in response to infection. The protein site-specific glycosylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpretation of relevant biological changes in response to infection.
Issue Date: 18-Aug-2023
Date of Acceptance: 27-Jun-2023
DOI: 10.1016/j.isci.2023.107257
ISSN: 2589-0042
Publisher: Elsevier
Journal / Book Title: iScience
Volume: 26
Issue: 8
Copyright Statement: © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (
Publication Status: Published
Conference Place: United States
Article Number: ARTN 107257
Online Publication Date: 2023-07-04
Appears in Collections:Department of Infectious Diseases

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