The application of the electron microscopes, the optical microscopes and the electron diffraction camera to the study of fine structure

Abstract

The limitation of the resolving power of the optical microscope is discussed and an account is given of the operation and construction of a phase contrast microscope which was found to be a valuable adjunct to the electron microscope.The electron microscope constructed in this laboratory and modified by the writer is described in outline, and the factors limiting its resolving power are discussed in detail. The formation of electron optical image with and without a limiting aperture in the objective lens is considered for object differing in mass thickness. The effect of Increasing the high tension voltage is discussed. The above optical and electron microscope were, applied to the study of the polished layer on glass surfaces and Sellby’s view on the flow of the polish layer were confirmed Myofibril from rat diaphragm were examined by phase cent root microscopy and certain changes in length and structure were observed in different states of activity produced by passive stretching and stimulation. Finally silver films evaporated in vacuo at different temperatures on to rocksalt cleavage faces were studied by means of electron microscopy and by electron diffraction but the electron micrographs added little to the knowledge previously obtained by the, in this case, far more informative technique of electron diffraction.