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3D sub-nanometer analysis of glucose in an aqueous solution by cryo-atom probe tomography

Title: 3D sub-nanometer analysis of glucose in an aqueous solution by cryo-atom probe tomography
Authors: Schwarz, TM
Dietrich, CA
Ott, J
Weikum, EM
Lawitzki, R
Solodenko, H
Hadjixenophontos, E
Gault, B
Kaestner, J
Schmitz, G
Stender, P
Item Type: Journal Article
Abstract: Atom Probe Tomography (APT) is currently a well-established technique to analyse the composition of solid materials including metals, semiconductors and ceramics with up to near-atomic resolution. Using an aqueous glucose solution, we now extended the technique to frozen solutions. While the mass signals of the common glucose fragments CxHy and CxOyHz overlap with (H2O)nH from water, we achieved stoichiometrically correct values via signal deconvolution. Density functional theory (DFT) calculations were performed to investigate the stability of the detected pyranose fragments. This paper demonstrates APT’s capabilities to achieve sub-nanometre resolution in tracing whole glucose molecules in a frozen solution by using cryogenic workflows. We use a solution of defined concentration to investigate the chemical resolution capabilities as a step toward the measurement of biological molecules. Due to the evaporation of nearly intact glucose molecules, their position within the measured 3D volume of the solution can be determined with sub-nanometre resolution. Our analyses take analytical techniques to a new level, since chemical characterization methods for cryogenically-frozen solutions or biological materials are limited.
Issue Date: 2-Jun-2021
Date of Acceptance: 18-May-2021
URI: http://hdl.handle.net/10044/1/104275
DOI: 10.1038/s41598-021-90862-8
ISSN: 2045-2322
Publisher: Nature Portfolio
Start Page: 1
End Page: 19
Journal / Book Title: Scientific Reports
Volume: 11
Issue: 1
Copyright Statement: © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Publication Status: Published
Article Number: ARTN 11607
Online Publication Date: 2021-06-02
Appears in Collections:Materials
Faculty of Engineering



This item is licensed under a Creative Commons License Creative Commons