A novel microfluidic point-of-care biosensor system on printed circuit board for cytokine detection
File(s)
Published version
Author(s)
Evans, Daniel
Papadimitriou, Konstantinos I
Vasilakis, Nikolaos
Pantelidis, Panagiotis
Kelleher, Peter
Type
Journal Article
Abstract
Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H2O2 depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.
Date Issued
2018-11-17
Date Acceptance
2018-11-12
Citation
Sensors, 2018, 18 (11)
ISSN
1424-2818
Publisher
MDPI AG
Journal / Book Title
Sensors
Volume
18
Issue
11
Copyright Statement
© 2018 The Author(s). This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Sponsor
Engineering & Physical Science Research Council (E
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000451598900421&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
RIS_13986/02; EP/L020920/1
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Analytical
Electrochemistry
Instruments & Instrumentation
Chemistry
cytokine detection
eELISA
lab-on-PCB
microfluidics
PCB biosensors
point-of-care diagnostics
PAPER-BASED DEVICE
INTERFERON-GAMMA
DIAGNOSTICS
TECHNOLOGIES
DISEASE
GOLD
AMPLIFICATION
IMMUNOASSAY
BIOMARKERS
PLATFORM
Publication Status
Published
Article Number
4011
Date Publish Online
2018-11-17