In this paper, a class of algorithms for automatic classification of individual musical instrument sounds is presented. Several perceptual features used in general sound classification applications were measured for 300 sound recordings consisting of 6 different musical instrument classes (piano, violin, cello, flute, bassoon and soprano saxophone). In addition, MPEG-7 basic spectral and spectral basis descriptors were considered, providing an effective combination for accurately describing the spectral and timbrai audio characteristics. The audio flies were split using 70% of the available data for training and the remaining 30% for testing. A classifier was developed based on non-negative matrix factorization (NMF) techniques, thus introducing a novel application of NMF. The standard NMF method was examined, as well as its modifications: the local, the sparse, and the discriminant NMF. Experimental results are presented to compare MPEG-7 spectral basis representations with MPEG-7 basic spectral features alongside the various NMF algorithms. The results indicate that the use of the spectrum projection coefficients for feature extraction and the standard NMF classifier yields an accuracy exceeding 95%. ©2006 IEEE.