Design of DNA strand displacement reactions
File(s)
Published version
Author(s)
Jurinović, Križan
Mitra, Merry
Mukherjee, Rakesh
Ouldridge, Thomas E
Type
Journal Article
Abstract
DNA strand displacement (SD) reactions are central to the operation of many synthetic nucleic acid systems, including molecular circuits, sensors, and machines. Over the years, a broad set of design frameworks has emerged to accommodate various functional goals, initial configurations, and environmental conditions. Nevertheless, key challenges persist, particularly in reliably predicting reaction kinetics. In contrast to reviews centred on network-level architectures, this article focuses on the design and analysis of individual SD reactions, highlighting kinetic mechanisms, structural determinants, and the current limits of predictive modelling. We identify promising innovations while analysing the factors that continue to hinder predictive accuracy. We conclude by outlining future directions for achieving more robust and programmable behaviour in DNA-based systems.
Date Issued
2026-02-01
Date Acceptance
2025-12-01
Citation
Current Opinion in Biotechnology, 2026, 97
ISSN
0958-1669
Publisher
Elsevier BV
Journal / Book Title
Current Opinion in Biotechnology
Volume
97
Copyright Statement
© 2025 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41349497
PII: S0958-1669(25)00140-5
Publication Status
Published
Coverage Spatial
England
Article Number
103396
Date Publish Online
2025-12-04