A facile method to quantify synthetic peptide concentrations on biomaterials
Author(s)
Type
Journal Article
Abstract
While it is well understood that peptides can greatly improve cell–material interactions, it is often challenging to determine the concentration of the peptide which decorates a material. Herein, we describe a straightforward method using readily, synthetically accessible Fmoc peptides and commercially available reagents to measure the concentration of peptides on nanoparticles, surfaces, and hydrogels. To achieve this, the Fmoc protecting group from immobilized peptides is removed under optimized basic conditions. The dibenzofulvene released can be quantified by HPLC or UV–vis spectroscopy, enabling a direct experimental measurement of the concentration of the peptide. We show that we can measure the concentration of a BMP-2 peptide mimic on a hydrogel to determine the concentration required to stimulate osteogenesis of human mesenchymal stem cells. We envision that this methodology will enable a more thorough understanding of the concentration of synthetic peptides decorated on many biomaterials (e.g., nanoparticles, surfaces, hydrogels) to improve deconvolution of the interactions at the cell–material interface.
Date Issued
2024-09-18
Date Acceptance
2024-08-23
Citation
ACS Applied Materials and Interfaces, 2024, 16 (37), pp.49880-49888
ISSN
1944-8244
Publisher
American Chemical Society
Start Page
49880
End Page
49888
Journal / Book Title
ACS Applied Materials and Interfaces
Volume
16
Issue
37
Copyright Statement
© 2024 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0 .
CC-BY 4.0 .
License URL
Identifier
https://pubs.acs.org/doi/10.1021/acsami.4c07164
Publication Status
Published
Date Publish Online
2024-09-09