Controlled delivery of microRNAs into primary cells using nanostraw technology
File(s)
Published version
Author(s)
Pop, Mara
Almquist, Benjamin
Type
Journal Article
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs that play key roles in posttranscriptional gene regulation. Being involved in regulating virtually all cellular processes, from proliferation and differentiation to migration and apoptosis, they have emerged as important epigenetic players. While most interest has gone into which miRNAs are involved in specific cellular processes or pathologies, the dosage-dependent effects of miRNAs remain vastly unexplored. Different doses of miRNAs can cause selective downregulation of target genes, in turn determining what signaling pathways and cellular responses are triggered. To explore this behavior, the effects of incremental miRNA dosage need to be studied; however, current delivery methods for miRNAs are unable to control how much miRNA enters a cell. Herein, an approach is presented based on a nanostraw–electroporation delivery platform that decouples the delivery from biological mechanisms (e.g., endocytosis) to enable precise control over the amount of miRNA delivered, along with demonstrating ratiometric intracellular delivery into primary dermal fibroblasts for miR-181a and miR-27a. In addition, it is shown that the nanostraw delivery platform allows efficient delivery of miRNAs into primary keratinocytes, opening new opportunities for successful miRNA delivery into this hard-to-transfect cell type.
Date Issued
2021-03-30
Date Acceptance
2021-03-22
Citation
Advanced NanoBiomed Research, 2021, 1 (6), pp.1-10
ISSN
2699-9307
Publisher
Wiley Open Access
Start Page
1
End Page
10
Journal / Book Title
Advanced NanoBiomed Research
Volume
1
Issue
6
License URL
© 2021 The Authors. Advanced NanoBiomed Research published by
Wiley-VCH GmbH. This is an open access article under the terms of
the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original
work is properly cited.
Sponsor
Wellcome Trust
Wellcome Trust
Rosetrees Trust
Rosetrees Trust
Diabetes Research & Wellness Foundation
Engineering and Physical Sciences Research Council
Identifier
https://onlinelibrary.wiley.com/doi/10.1002/anbr.202000061
Grant Number
109838/Z/15/Z
109838/Z/15/Z
A1071
A1071
1770
EP/L504786/1
Subjects
controlled delivery
epigenetics
microRNA
nanobiotechnology
nanostraw
Publication Status
Published
Article Number
ARTN 2000061
Date Publish Online
2021-03-30