Comparison of the effects of 45S5 and 1393 bioactive glass microparticles on hMSC behavior
Author(s)
Type
Journal Article
Abstract
Bioactive glasses (BAGs) are highly interesting materials for bone regeneration applications in orthopedic and dental defects. It is quite well known that ionic release from BAGs influences cell behavior and function. Mindful of the clinical scenario, we hypothesized that local cell populations might additionally physically interact with the implanted BAG particles and respond differently than to just the ionic stimuli. We therefore studied the biological effect of two BAG types (45S5 and 1393) applied to human mesenchymal stromal cells (hMSCs) in three distinct presentation modes: (a) direct contact; and to dissolution products in (b) 2D, and (c) 3D culture. We furthermore investigated how the dose-dependence of these BAG particles, in concentrations ranging from 0.1 to 2.5 w/v %, influenced hMSC metabolic activity, proliferation, and cell spreading. These cellular functions were significantly hampered when hMSCs were exposed to high concentrations of either glasses, but the effects were more pronounced in the 45S5 groups and when the cells were in direct contact with the BAGs. Furthermore the biological effect of 1393 BAG outperformed that of 45S5 BAG in all tested presentation modes. These outcomes highlight the importance of investigating cell–BAG interactions in experimental set-ups that recapitulate host cell interactions with BAG particles.
Date Issued
2017-07-06
Date Acceptance
2017-05-26
ISSN
1549-3296
Publisher
Wiley
Start Page
2772
End Page
2782
Journal / Book Title
Journal of Biomedical Materials Research Part A
Volume
105
Issue
10
Copyright Statement
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Subjects
Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
bioactive glass
hMSCs
microparticles
cell function
ion release
MESENCHYMAL STEM-CELLS
IN-VITRO
CONTROLLABLE DEGRADATION
MACROPHAGE RESPONSES
COMPOSITE SCAFFOLDS
BONE REGENERATION
BIOGLASS(R)
SILICATE
ANGIOGENESIS
BOROSILICATE
09 Engineering
06 Biological Sciences
Publication Status
Published