Pericyte seeded dual peptide scaffold with improved endothelialization for vascular graft tissue engineering
File(s)
Author(s)
Type
Journal Article
Abstract
The development of synthetic vascular grafts for coronary artery bypass is challenged by insufficient endothelialization, which exposes to the risk of thrombosis, and lack of native cellular constituents, which favours pathological remodelling. Here, an bifunctional electrospun poly(ε-caprolactone) (PCL) scaffold with potential for synthetic vascular graft applications is presented. This scaffold incorporates two tethered peptides: the osteopontin-derived peptide (Adh) on the ‘luminal’ side and a heparin-binding peptide (Hep) on the ‘abluminal’ side. Additionally, the ‘abluminal’ side of the scaffold is seeded with saphenous vein-derived pericytes (SVPs) as a source of pro-angiogenic growth factors. The Adh peptide significantly increase endothelial cell adhesion, while the Hep peptide promote accumulation of vascular endothelial growth factor (VEGF) secreted by SVPs. SVPs increase endothelial migration both in a transwell assay and a modified scratch assay performed on the PCL scaffold. Seeding of SVPs on the ‘abluminal’/Hep side of the scaffold further increase endothelial cell density, indicating a combinatory effect of the peptides and pericytes. Lastly, SVP-seeded scaffolds are preserved by freezing in a xeno-free medium, maintaining good cell viability and function. In conclusion, this engineered scaffold combines patient-derived pericytes and spatially organized functionalities, which synergistically increase endothelial cell density and growth factor retention.
Date Issued
2016-10-26
Date Acceptance
2016-09-27
Citation
Advanced Healthcare Materials, 2016, 5 (23), pp.3046-3055
ISSN
2192-2640
Publisher
Wiley
Start Page
3046
End Page
3055
Journal / Book Title
Advanced Healthcare Materials
Volume
5
Issue
23
Copyright Statement
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the accepted version of the following article: Campagnolo, P.et al., (2016), Pericyte Seeded Dual Peptide Scaffold with Improved Endothelialization for Vascular Graft Tissue Engineering. Adv. Healthcare Mater., 5: 3046–3055., which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/adhm.201600699/abstract
Sponsor
Commission of the European Communities
Medical Research Council (MRC)
Grant Number
ERC-2013-CoG-616417
RA26F7
Subjects
biofunctionalization
electrospinning
endothelialization
pericytes
tissue engineered vascular graft
Publication Status
Published