microRNA expression in women with and without polycystic ovarian syndrome matched for body mass index
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Author(s)
Type
Journal Article
Abstract
Background: Despite several authors who have hypothesized that alterations of small
noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian
syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body
mass index (BMI) levels may account for these differences; therefore, the aim of the
present study was to determine whether miR differed in serum samples collected from
age- and BMI-matched control and PCOS women.
Methods: In a cross-sectional study, miR were measured using quantitative polymerase
chain reaction in 29 women with anovulatory PCOS women and 29 control women who
were in the follicular phase of their menstrual cycle, from the local biobank.
Results: One hundred seventy-six miR were detected, of which 15 miR passed the false
discovery rate (FDR; p < 0.05) that differed between PCOS and control women. There
was no association of the top 9 miR (p < 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p,
miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with
BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either
PCOS or normal women. Ingenuity pathway assessment showed the pathways were
interrelated for abnormalities of the reproductive system.
Conclusion: When the confounding influence of weight was accounted for, miR levels
differed between anovulatory PCOS women and control women in the follicular phase of
the menstrual cycle. Interestingly, the differing miR were associated with the pathways of
reproductive abnormalities but did not associate with AMH or metabolic parameters.
noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian
syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body
mass index (BMI) levels may account for these differences; therefore, the aim of the
present study was to determine whether miR differed in serum samples collected from
age- and BMI-matched control and PCOS women.
Methods: In a cross-sectional study, miR were measured using quantitative polymerase
chain reaction in 29 women with anovulatory PCOS women and 29 control women who
were in the follicular phase of their menstrual cycle, from the local biobank.
Results: One hundred seventy-six miR were detected, of which 15 miR passed the false
discovery rate (FDR; p < 0.05) that differed between PCOS and control women. There
was no association of the top 9 miR (p < 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p,
miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with
BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either
PCOS or normal women. Ingenuity pathway assessment showed the pathways were
interrelated for abnormalities of the reproductive system.
Conclusion: When the confounding influence of weight was accounted for, miR levels
differed between anovulatory PCOS women and control women in the follicular phase of
the menstrual cycle. Interestingly, the differing miR were associated with the pathways of
reproductive abnormalities but did not associate with AMH or metabolic parameters.
Date Issued
2020-04-28
Date Acceptance
2020-03-24
Citation
Frontiers in Endocrinology, 2020, 11, pp.1-8
ISSN
1664-2392
Publisher
Frontiers Media
Start Page
1
End Page
8
Journal / Book Title
Frontiers in Endocrinology
Volume
11
Copyright Statement
© 2020 Butler, Ramachandran, Sathypalan, David, Gooderham,
Benurwar, Dargham, Hayat, Hani Najafi-Shoushtari and Atkin. This is an openaccess article distributed under the terms of the Creative Commons Attribution
License (CC BY). The use, distribution or reproduction in other forums is permitted,
provided the original author(s) and the copyright owner(s) are credited and that the
original publication in this journal is cited, in accordance with accepted academic
practice. No use, distribution or reproduction is permitted which does not comply
with these terms.
Benurwar, Dargham, Hayat, Hani Najafi-Shoushtari and Atkin. This is an openaccess article distributed under the terms of the Creative Commons Attribution
License (CC BY). The use, distribution or reproduction in other forums is permitted,
provided the original author(s) and the copyright owner(s) are credited and that the
original publication in this journal is cited, in accordance with accepted academic
practice. No use, distribution or reproduction is permitted which does not comply
with these terms.
Identifier
https://www.frontiersin.org/articles/10.3389/fendo.2020.00206/full
Subjects
1103 Clinical Sciences
1111 Nutrition and Dietetics
Publication Status
Published online
Date Publish Online
2020-04-28