In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Imaging techniques for quantifying changes in the hierarchical structure of deforming joints are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast low-dose pink-beam synchrotron X-ray tomography in combination with mechanical loading at nanometric precision for in situ imaging, at resolutions below 100 nm, of the mechanical strain in intact untreated joints under physiologically realistic conditions. We show that in young, older and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that the tissue structure at the cellular level correlates with the mechanical performance of the whole joint. We also use the tomographic approach to study the colocalization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints and to explore the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.
Date Issued
2020-03-01
Date Acceptance
2019-10-11
Citation
Nature Biomedical Engineering, 2020, 4, pp.343-354
ISSN
2157-846X
Publisher
Nature Research
Start Page
343
End Page
354
Journal / Book Title
Nature Biomedical Engineering
Volume
4
Copyright Statement
© 2019 The Author(s), under exclusive licence to Springer Nature Limited
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31768001
PII: 10.1038/s41551-019-0477-1
Subjects
Science & Technology
Technology
Engineering, Biomedical
Engineering
ARTICULAR CALCIFIED CARTILAGE
DIGITAL VOLUME CORRELATION
SCANNING-ELECTRON-MICROSCOPY
SUBCHONDRAL BONE PLATE
CORTICAL BONE
MECHANICAL-PROPERTIES
FEMORAL-HEAD
OSTEOARTHRITIS
MICROSTRUCTURE
DEFORMATION
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2019-11-25