Characterization of a unique repression system present in arbitrium phages of the SPbeta family
File(s)
Author(s)
Brady, Aisling
Cabello-Yeves, Elena
Gallego Del Sol, Francisca
Chmielowska, Cora
Mancheño-Bonillo, Javier
Type
Journal Article
Abstract
Arbitrium-coding phages use peptides to communicate and coordinate the decision between lysis and lysogeny. However, the mechanism by which these phages establish lysogeny remains unknown. Here, focusing on the SPbeta phage family's model phages phi3T and SPβ, we report that a six-gene operon called the "SPbeta phages repressor operon" (sro) expresses not one but two master repressors, SroE and SroF, the latter of which folds like a classical phage integrase. To promote lysogeny, these repressors bind to multiple sites in the phage genome. SroD serves as an auxiliary repressor that, with SroEF, forms the repression module necessary for lysogeny establishment and maintenance. Additionally, the proteins SroABC within the operon are proposed to constitute the transducer module, connecting the arbitrium communication system to the activity of the repression module. Overall, this research sheds light on the intricate and specialized repression system employed by arbitrium SPβ-like phages in making lysis-lysogeny decisions.
Date Issued
2023-12-13
Date Acceptance
2023-11-03
Citation
Cell Host and Microbe, 2023, 31 (12), pp.2023-2037.e8
ISSN
1931-3128
Publisher
Cell Press
Start Page
2023
End Page
2037.e8
Journal / Book Title
Cell Host and Microbe
Volume
31
Issue
12
Copyright Statement
© 2023 The Author(s). Published by Elsevier Inc. 2023
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38035880
PII: S1931-3128(23)00453-5
Subjects
Bacteriophages
Lysogeny
Peptides
arbitrium
communication
lysis
lysogeny
MazEF
quorum sensing
repressor
SOS response
toxin-antitoxin
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2023-11-29