Functional roles of the pre-sensor I insertion sequence in an AAA plus bacterial enhancer binding protein
Author(s)
Type
Journal Article
Abstract
Molecular machines belonging to the AAA+ superfamily of ATPases use NTP hydrolysis to remodel their versatile substrates. The presence of an insertion sequence defines the major phylogenetic pre‐sensor I insertion (pre‐SIi) AAA+ superclade. In the bacterial σ54‐dependent enhancer binding protein phage shock protein F (PspF) the pre‐SIi loop adopts different conformations depending on the nucleotide‐bound state. Single amino acid substitutions within the dynamic pre‐SIi loop of PspF drastically change the ATP hydrolysis parameters, indicating a structural link to the distant hydrolysis site. We used a site‐specific protein–DNA proximity assay to measure the contribution of the pre‐SIi loop in σ54‐dependent transcription and demonstrate that the pre‐SIi loop is a major structural feature mediating nucleotide state‐dependent differential engagement with Eσ54. We suggest that much, if not all, of the action of the pre‐SIi loop is mediated through the L1 loop and relies on a conserved molecular switch, identified in a crystal structure of one pre‐SIi variant and in accordance with the high covariance between some pre‐SIi residues and distinct residues outside the pre‐SIi sequence.
Date Issued
2009-08-01
Online Publication Date
2020-02-14T10:08:54Z
Date Acceptance
2009-05-11
ISSN
0950-382X
Publisher
Wiley
Start Page
519
End Page
533
Journal / Book Title
Molecular Microbiology
Volume
73
Issue
4
Copyright Statement
© 2009 Blackwell Publishing Ltd. No claim to original US government works.
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2958.2009.06744.x
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000268793000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
BB/G020434/1
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Microbiology
PROMOTER DNA COMPLEX
RNA-POLYMERASE
ALUMINUM FLUORIDE
CRYSTAL-STRUCTURE
ESCHERICHIA-COLI
STRUCTURAL BASIS
PORE LOOPS
TRANSCRIPTION
HYDROLYSIS
DOMAIN
Adenosine Triphosphatases
Adenosine Triphosphate
Amino Acid Substitution
Escherichia coli
Escherichia coli Proteins
Hydrolysis
Mutagenesis, Insertional
Protein Structure, Tertiary
RNA Polymerase Sigma 54
Trans-Activators
Transcriptional Activation
Escherichia coli
Escherichia coli Proteins
Trans-Activators
Adenosine Triphosphate
Amino Acid Substitution
Mutagenesis, Insertional
Protein Structure, Tertiary
Hydrolysis
RNA Polymerase Sigma 54
Adenosine Triphosphatases
Transcriptional Activation
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Microbiology
PROMOTER DNA COMPLEX
RNA-POLYMERASE
ALUMINUM FLUORIDE
CRYSTAL-STRUCTURE
ESCHERICHIA-COLI
STRUCTURAL BASIS
PORE LOOPS
TRANSCRIPTION
HYDROLYSIS
DOMAIN
Microbiology
06 Biological Sciences
11 Medical and Health Sciences
07 Agricultural and Veterinary Sciences
Publication Status
Published
Date Publish Online
2009-05-26