Poly(ethylene glycol)-containing hydrogels promote the release of primary granules from human blood-derived polymorphonuclear leukocytes
File(s)nihms-584594.pdf (761.91 KB)
Accepted version
Author(s)
Cohen, HC
Lieberthal, TJ
Kao, WJ
Type
Journal Article
Abstract
Polymorphonuclear leukocytes (PMNs) are recruited to sites of injury and biomaterial implants. Once activated, PMNs can exocytose their granule subsets to recruit monocytes (MCs) and mediate MC/macrophage activation. We investigated the release of myeloperoxidase (MPO), a primary granule marker, and matrix metalloproteinase-9 (MMP-9), a tertiary granule marker, from human blood-derived PMNs cultured on poly(ethylene glycol) (PEG) hydrogels, polydimethylsiloxane (PDMS), tissue culture polystyrene (TCPS) and gelatin–PEG (GP) hydrogels, with and without the presence of the bacterial peptide formyl-Met-Leu-Phe. Supernatants from PMN cultures on PEG-containing hydrogels (i.e., PEG and GP hydrogels) had higher concentrations of MPO than those from PMN cultures on PDMS or TCPS at 2 h. PMNs on all biomaterials released comparable levels of MMP-9 at 2 h, indicating that PMNs cultured on PEG-containing hydrogels have different mechanisms of release for primary and tertiary granules. Src family kinases were involved in the release of MPO from PMNs cultured on PEG hydrogels, TCPS and GP hydrogels and in the release of MMP-9 from PMNs cultured on all four biomaterials. The increased release of primary granules from PMNs on PEG-containing hydrogels did not significantly increase MC chemotaxis, indicating that additional co-effectors in the dynamic inflammatory milieu in vivo modulate PMN-mediated MC recruitment.
Date Issued
2014-02-13
Online Publication Date
2014-02-13
2016-09-20T09:12:12Z
Date Acceptance
2014-01-29
ISSN
1552-4965
Publisher
Wiley
Start Page
4252
End Page
4261
Journal / Book Title
Journal of Biomedical Materials Research Part A
Volume
102
Issue
12
Copyright Statement
© 2014 Wiley Periodicals, Inc. This is the accepted version of the following article: Cohen HC, Lieberthal TJ, Kao WJ. 2014. Poly(ethylene glycol)-containing hydrogels promote the release of primary granules from human blood-derived polymorphonuclear leukocytes. J Biomed Mater Res Part A 2014:102A:4252–4261, which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35101.
Source Database
crossref
Subjects
Neutrophil
acute inflammation
degranulation
matrix metalloproteinase-9
myeloperoxidase
poly(ethylene glycol)
Chemotaxis
Culture Media, Conditioned
Humans
Hydrogels
Macrophage Activation
Macrophages
Matrix Metalloproteinase 9
Monocytes
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils
Polyethylene Glycols
Secretory Vesicles
09 Engineering
06 Biological Sciences
Publication Status
Published