Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animals.
Author(s)
Chaban, B
Ngeleka, M
Hill, JE
Type
Journal Article
Abstract
BACKGROUND: The genus Campylobacter includes many species, some of which are known human and animal pathogens. Even though studies have repeatedly identified domestic dogs as a risk factor for human campylobacteriosis, our understanding of Campylobacter ecology in this reservoir is limited. Work to date has focused primarily on a limited number of species using culture-based methods. To expand our understanding of Campylobacter ecology in dogs, a collection of fecal samples from 70 healthy and 65 diarrheic pet dogs were examined for the presence and levels of 14 Campylobacter species using quantitative PCR. RESULTS: It was found that 58% of healthy dogs and 97% of diarrheic dogs shed detectable levels of Campylobacter spp., with C. coli, C. concisus, C. fetus, C. gracilis, C. helveticus, C. jejuni, C. lari, C. mucosalis, C. showae, C. sputorum and C. upsaliensis levels significantly higher in the diarrheic population. Levels of individual Campylobacter species detected ranged from 103 to 108 organisms per gram of feces. In addition, many individual samples contained multiple species of Campylobacter, with healthy dogs carrying from 0-7 detectable species while diarrheic dogs carried from 0-12 detectable species. CONCLUSIONS: These findings represent the largest number of Campylobacter species specifically tested for in animals and is the first report to determine quantifiable levels of Campylobacter being shed from dogs. This study demonstrates that domestic dogs can carry a wide range of Campylobacter species naturally and that there is a notable increase in species richness detectable in the diarrheic population. With several of the detected Campylobacter species known or emerging pathogens, these results are relevant to both ecological and public health discussions.
Date Issued
2010-03-10
Date Acceptance
2010-03-10
Citation
BMC Microbiology, 2010, 10
ISSN
1471-2180
Publisher
BioMed Central
Journal / Book Title
BMC Microbiology
Volume
10
Copyright Statement
© 2010 Chaban et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Subjects
Animals
Animals, Domestic
Bacterial Shedding
Campylobacter
Diarrhea
Dog Diseases
Dogs
Feces
Genes, Bacterial
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Statistics, Nonparametric
Publication Status
Published
Article Number
73