Engineering anisotropic muscle tissue using acoustic cell patterning
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.
Date Issued
2018-10-25
Date Acceptance
2018-08-09
Citation
Advanced Materials, 2018, 30 (43), pp.1-7
ISSN
0935-9648
Publisher
Wiley
Start Page
1
End Page
7
Journal / Book Title
Advanced Materials
Volume
30
Issue
43
Sponsor
Commission of the European Communities
British Heart Foundation
Medical Research Council (MRC)
Medical Research Council (MRC)
Medical Research Council (MRC)
Commission of the European Communities
Wellcome Trust
Medical Research Council (MRC)
Arthritis Research UK
Medical Research Council
Arthritis Research UK
Identifier
https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201802649
Grant Number
701664
RM/13/1/30157
MR/K026682/1
MR/R015651/1
MR/L012677/1
ERC-2013-CoG-616417
098411/Z/12/Z
MR/S00551X/1
21138
MR/S00551X/1
21138
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
acoustic
muscle
patterning
tissue engineering
ultrasound standing waves
SKELETAL-MUSCLE
IN-VITRO
CONTRACTION
HYDROGELS
acoustic
muscle
patterning
tissue engineering
ultrasound standing waves
Acoustics
Animals
Cell Line
Collagen
Hydrogels
Mice
Muscle Fibers, Skeletal
Myoblasts
Tissue Engineering
Tissue Scaffolds
Ultrasonic Waves
Cell Line
Myoblasts
Animals
Mice
Collagen
Hydrogels
Tissue Engineering
Acoustics
Tissue Scaffolds
Muscle Fibers, Skeletal
Ultrasonic Waves
Nanoscience & Nanotechnology
02 Physical Sciences
03 Chemical Sciences
09 Engineering
Publication Status
Published
Article Number
1802649
Date Publish Online
2018-09-12