The RNA Helicase eIF4A Is Required for Sapovirus Translation

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The RNA Helicase eIF4A Is Required for Sapovirus Translation.pdf (1.37 MB)

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OA Location

http://jvi.asm.org/content/90/10/5200.full

Author(s)

Hosmillo, M

Sweeney, TR

Chaudhry, Y

Leen, E

Curry, S

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Type

Journal Article

Abstract

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5′ untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5′ UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.

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Date Issued

2016-03-02

Date Acceptance

2016-02-26

Citation

Journal of Virology, 2016, 90 (10), pp.5200-5204

URI

http://hdl.handle.net/10044/1/34220

DOI

https://www.dx.doi.org/10.1128/JVI.03174-15

ISSN

1098-5514

Publisher

American Society for Microbiology

Start Page

5200

End Page

5204

Journal / Book Title

Journal of Virology

Volume

90

Issue

10

Copyright Statement

© 2016 Hosmillo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

License URL

http://creativecommons.org/licenses/by/4.0/

Sponsor

Biotechnology and Biological Sciences Research Council (BBSRC)

Grant Number

BB/J001708/1

Subjects

Science & Technology

Life Sciences & Biomedicine

Virology

PORCINE ENTERIC CALICIVIRUS

MESSENGER-RNAS

INITIATION

VPG

BINDING

06 Biological Sciences

07 Agricultural And Veterinary Sciences

11 Medical And Health Sciences

Publication Status

Published