Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror
File(s)Wright_2021_J._Phys._Photonics_3_045004.pdf (4.42 MB)
Published version
Author(s)
Wright, terry
Sparks, hugh
Paterson, carl
Dunsby, Christopher
Type
Journal Article
Abstract
This paper presents the use of a deformable mirror (DM) configured to rapidly refocus a microscope employing a high
numerical aperture objective lens. An Alpao DM97-15 membrane DM was used to refocus a 40×/0.80 NA water-immersion
objective through a defocus range of -50 to 50 m at 26.3 sweeps per second. We achieved imaging with a mean Strehl
metric of > 0.6 over a field of view in the sample of 200×200 m2 over a defocus range of 77 m. We describe an
optimisation procedure where the mirror is swept continuously in order to avoid known problems of hysteresis associated
with the membrane DM employed. This work demonstrates that a DM-based refocusing system could in the future be used in
light-sheet fluorescence microscopes to achieve video-rate volumetric imaging.
numerical aperture objective lens. An Alpao DM97-15 membrane DM was used to refocus a 40×/0.80 NA water-immersion
objective through a defocus range of -50 to 50 m at 26.3 sweeps per second. We achieved imaging with a mean Strehl
metric of > 0.6 over a field of view in the sample of 200×200 m2 over a defocus range of 77 m. We describe an
optimisation procedure where the mirror is swept continuously in order to avoid known problems of hysteresis associated
with the membrane DM employed. This work demonstrates that a DM-based refocusing system could in the future be used in
light-sheet fluorescence microscopes to achieve video-rate volumetric imaging.
Date Issued
2021-10-20
Date Acceptance
2021-09-23
Citation
Journal of Physics: Photonics, 2021, 3 (4), pp.1-14
ISSN
2515-7647
Publisher
IOP Publishing
Start Page
1
End Page
14
Journal / Book Title
Journal of Physics: Photonics
Volume
3
Issue
4
Copyright Statement
© 2021 The Author(s). Published by IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 4.0 license. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
License URL
Sponsor
British Heart Foundation
Identifier
https://iopscience.iop.org/article/10.1088/2515-7647/ac29a2
Grant Number
NH/16/1/32447
Subjects
Science & Technology
Physical Sciences
Optics
adaptive optics
remote refocusing
light-sheet fluorescence microscopy
deformable mirror
ADAPTIVE OPTICS
EXTENDED DEPTH
MICROSCOPY
STABILITY
adaptive optics
deformable mirror
light-sheet fluorescence microscopy
remote refocusing
Publication Status
Published
Date Publish Online
2021-10-20