Bioenergetic Changes during Differentiation of Human Embryonic Stem Cells along the Hepatic Lineage.
Author(s)
Type
Journal Article
Abstract
Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. Nevertheless, a full understanding of the role of mitochondrial bioenergetics through differentiation is still lacking. Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation.
Date Issued
2017-02-06
Online Publication Date
2017-02-06
2017-09-04T12:26:56Z
Date Acceptance
2017-01-10
ISSN
1942-0900
Publisher
Hindawi Publishing Corporation
Journal / Book Title
Oxidative Medicine and Cellular Longevity
Volume
2017
Copyright Statement
Copyright © 2017 Branden M. Hopkinson et al. This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
Identifier
https://www.hindawi.com/journals/omcl/2017/5080128/
Subjects
Science & Technology
Life Sciences & Biomedicine
Cell Biology
MITOCHONDRIAL
LIVER
GLYCOLYSIS
EXPRESSION
GENERATION
PHENOTYPES
MUSCLE
HEART
MICE
Cell Differentiation
Cell Line
Cell Respiration
Energy Metabolism
Fluorescent Antibody Technique, Indirect
Hepatocytes
Human Embryonic Stem Cells
Humans
Microscopy, Confocal
Mitochondria
Polymerase Chain Reaction
Publication Status
Published
Country
United States
Article Number
5080128