A highly efficient multi-step methodology for the quantification of micro-(bio)plastics in sludge.
File(s)
Author(s)
Type
Journal Article
Abstract
The present study develops a multi-step methodology for identification and quantification of microplastics and micro-bioplastics (together called in the current work micro-(bio)plastics) in sludge. In previous studies, different methods for the extraction of microplastics were devised for traditional plastics, while the current research tested the methodology on starch-based micro-bioplastics of 0.1-2 mm size. Compostable bioplastics are expected to enter the anaerobic or aerobic biological treatments that lead to end-products applicable in agriculture; some critical conditions of treatments (e.g. low temperature and moisture) can slow down the degradation process and be responsible for the presence of microplastics in the end-product. The methodology consists of an initial oxidation step, with hydrogen peroxide 35% concentrated to clear the sludge and remove the organic fraction, followed by a combination of flotation with sodium chloride and observation of the residues under a fluorescence microscope using a green filter. The workflow revealed an efficacy of removal from 94% to 100% and from 92% to 96% for plastic fragments, 0.5-2 mm and 0.1-0.5 mm size, respectively. The methodology was then applied to samples of food waste pulp harvested after a shredding pre-treatment in an anaerobic digestion (AD) plant in Italy, where polyethylene, starch-based Mater-Bi® and cellophane microplastics were recovered in amounts of 9 ± 1.3/10 g <2 mm and 4.8 ± 1.2/10 g ⩾2 mm. The study highlights the need to lower the threshold size for the quantification of plastics in organic fertilizers, which is currently set by legislations at 2 mm, by improving the background knowledge about the fate of the micro-(bio)plastics in biological treatments for the organic waste.
Date Issued
2020-11-29
Date Acceptance
2020-10-26
Citation
Waste Management and Research, 2020, 39 (7), pp.956-965
ISSN
0734-242X
Publisher
SAGE Publications
Start Page
956
End Page
965
Journal / Book Title
Waste Management and Research
Volume
39
Issue
7
Copyright Statement
© The Author(s) 2020. The final, definitive version of this paper has been published in Ruggero F, Porter AE, Voulvoulis N, et al. A highly efficient multi-step methodology for the quantification of micro-(bio)plastics in sludge. Waste Management & Research. November 2020 by Sage Publications Ltd. All rights reserved. It is available at: https://doi.org/10.1177/0734242X20974094
Sponsor
Natural Environment Research Council (NERC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33250042
Grant Number
NE/N006402/1
Subjects
Microplastics
bioplastics
extraction
methodology
quantification
regulations
sludge
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2020-11-29