Protein crystal occurrence domains in selective protein crystallisation for bio-separation
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Published version
Author(s)
Li, Xiaoyu
Chen, Wenqian
Yang, Huaiyu
Yang, Zhongqiang
Heng, Jerry YY
Type
Journal Article
Abstract
Bio-separation is a key bottleneck in the manufacture of biopharmaceuticals. In this work, we report experimental evidence of direct selective protein crystallisation from a binary protein mixture solution. Lysozyme–thaumatin mixtures with a wide protein composition range (0–100 mg mL−1, respectively) were tested under the same crystallisation cocktail conditions using the hanging-drop vapour-diffusion (HDVD) crystallisation method. This work demonstrates the selectivity of crystallisation from a model binary protein mixture and four crystal occurrence domains were determined as the operation windows of selective crystallisation of the target protein: 1) an unsaturated region with no crystal formation, 2 & 3) target regions with only a single type of protein crystals (lysozyme crystals only or thaumatin crystals only) and 4) a mixture region which have a mixture of both types of protein. This study demonstrates that protein crystallisation is not only applicable to high-purity protein solutions and emphasizes the vital impacts of the presence of protein impurities in the process of target protein crystallisation. The study concludes that protein crystallisation is a feasible approach to separate a target protein from a complex mixture environment which can be achieved by manipulating the crystallisation operation conditions such as mixture composition, precipitant concentration, and operation time.
Date Issued
2020-07-21
Date Acceptance
2020-06-02
Citation
CrystEngComm, 2020, 22 (27), pp.4566-4572
ISSN
1466-8033
Publisher
Royal Society of Chemistry
Start Page
4566
End Page
4572
Journal / Book Title
CrystEngComm
Volume
22
Issue
27
Copyright Statement
© The Royal Society of Chemistry 2020. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence http://creativecommons.org/licenses/by/3.0/.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000549313300004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Crystallography
Chemistry
PURIFICATION
PRECIPITATION
DELIVERY
PLATFORM
Publication Status
Published
Date Publish Online
2020-06-02