Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the industrial bacterium Corynebacterium glutamicum has not been explored. Here, we report the formation of liquid protein condensates in C. glutamicum using the RGG domain of Caenorhabditis elegans LAF-1. We optimized conditions for condensate formation, including the pre-induction period, inducer concentration, and cultivation temperature. Using the indigoidine biosynthesis pathway as a model, we demonstrated that LLPS-mediated MLOs enhanced indigoidine production. Furthermore, we applied these MLOs to modulate the toxicity of antimicrobial peptides (AMPs) to host cells, facilitating the expression of AMPs, including melittin and lactoferricin B. These findings provide insights into MLOs engineering in C. glutamicum and suggest broader applications of LLPS-mediated systems in industrial biotechnology.
Date Issued
2025-12-01
Date Acceptance
2025-08-01
Citation
Synthetic and Systems Biotechnology, 2025, 10 (4), pp.1331-1340
ISSN
2405-805X
Publisher
KeAi Communications Co., Ltd.
Start Page
1331
End Page
1340
Journal / Book Title
Synthetic and Systems Biotechnology
Volume
10
Issue
4
Copyright Statement
© 2025 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40893468
PII: S2405-805X(25)00112-7
Subjects
Antimicrobial peptides
Corynebacterium glutamicum
Indigoidine
liquid−liquid phase separation
Membraneless compartment
Publication Status
Published
Coverage Spatial
China
Date Publish Online
2025-08-05