Spatial Distribution of the Cannabinoid Type 1 and Capsaicin Receptors May Contribute to the Complexity of Their Crosstalk
File(s)
Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
The cannabinoid type 1 (CB1) receptor and the capsaicin receptor (TRPV1) exhibit
co-expression and complex, but largely unknown, functional interactions in a subpopulation
of primary sensory neurons (PSN). We report that PSN co-expressing CB1
receptor and TRPV1 form two distinct sub-populations based on their
pharmacological properties, which could be due to the distribution pattern of the two
receptors. Pharmacologically, neurons respond either only to capsaicin (COR
neurons) or to both capsaicin and the endogenous TRPV1 and CB1 receptor ligand
anandamide (ACR neurons). Blocking or deleting the CB1 receptor only reduces both
anandamide- and capsaicin-evoked responses in ACR neurons. Deleting the CB1
receptor also reduces the proportion of ACR neurons without any effect on the overall
number of capsaicin-responding cells. Regarding the distribution pattern of the two
receptors, neurons express CB1 and TRPV1 receptors either isolated in low densities
or in close proximity with medium/high densities. We suggest that spatial distribution
of the CB1 receptor and TRPV1 contributes to the complexity of their functional
interaction.
co-expression and complex, but largely unknown, functional interactions in a subpopulation
of primary sensory neurons (PSN). We report that PSN co-expressing CB1
receptor and TRPV1 form two distinct sub-populations based on their
pharmacological properties, which could be due to the distribution pattern of the two
receptors. Pharmacologically, neurons respond either only to capsaicin (COR
neurons) or to both capsaicin and the endogenous TRPV1 and CB1 receptor ligand
anandamide (ACR neurons). Blocking or deleting the CB1 receptor only reduces both
anandamide- and capsaicin-evoked responses in ACR neurons. Deleting the CB1
receptor also reduces the proportion of ACR neurons without any effect on the overall
number of capsaicin-responding cells. Regarding the distribution pattern of the two
receptors, neurons express CB1 and TRPV1 receptors either isolated in low densities
or in close proximity with medium/high densities. We suggest that spatial distribution
of the CB1 receptor and TRPV1 contributes to the complexity of their functional
interaction.
Date Issued
2016-09-22
Date Acceptance
2016-08-24
Citation
Scientific Reports, 2016, 6
ISSN
2045-2322
Publisher
Nature Publishing Group
Journal / Book Title
Scientific Reports
Volume
6
Copyright Statement
This work is licensed under a Creative Commons Attribution 4.0 International License. The images
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material. To view a copy of this
license, visit http://creativecommons.org/licenses/by/4.0/
© The Author(s) 2016
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material. To view a copy of this
license, visit http://creativecommons.org/licenses/by/4.0/
© The Author(s) 2016
License URL
Sponsor
Commission of the European Communities
Grant Number
254661
Publication Status
Published
Article Number
33307