Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion
Author(s)
Type
Journal Article
Abstract
Problem
Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first‐trimester trophoblast (HTR8) cells.
Method of study
HTR‐8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)‐8 and IL‐6.
Results
VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR‐8 invasion. The effects on invasion were blocked by TLR‐4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression.
Conclusions
VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not.
Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM− and VT−/PM+ subjects on human first‐trimester trophoblast (HTR8) cells.
Method of study
HTR‐8 cells were incubated with APS VT+/PM−, APS VT−/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)‐8 and IL‐6.
Results
VT−/PM+ IgG, but not VT+/PM− IgG significantly reduced HTR‐8 invasion. The effects on invasion were blocked by TLR‐4 inhibition. Neither VT+/PM− nor VT−/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression.
Conclusions
VT−/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM− IgG do not.
Date Issued
2015-05-01
Date Acceptance
2014-10-28
Citation
American Journal of Reproductive Immunology, 2015, 73 (5), pp.390-401
ISSN
1046-7408
Publisher
Wiley
Start Page
390
End Page
401
Journal / Book Title
American Journal of Reproductive Immunology
Volume
73
Issue
5
Copyright Statement
© 2014 The Authors. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352810200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Immunology
Reproductive Biology
Antiphospholipid
obstetric
TLR4
trophoblast
SYSTEMIC-LUPUS-ERYTHEMATOSUS
1ST TRIMESTER TROPHOBLAST
SIGNALING PATHWAYS
FACTOR EXPRESSION
ANTIBODIES
MONOCYTES
HEPARIN
DOMAIN
MULTICENTER
TLR4/MYD88
Publication Status
Published
Date Publish Online
2014-12-02