Rationalisation and validation of an acrylamide-free procedure in three-dimensional histological imaging
File(s)
Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
Three-dimensional visualization of intact tissues is now being achieved by turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to achieve optical transparency. To preserve tissue integrity, an acrylamide-based hydrogel has been proposed to embed the tissue. In this study, we examined the rationale behind the use of acrylamide in CLARITY, and presented evidence to suggest that the omission of acrylamide-hydrogel embedding in CLARITY does not alter the preservation of tissue morphology and molecular information in fixed tissues. We therefore propose a novel and simplified workflow for formaldehyde-fixed tissue clearing, which will facilitate the laboratory implementation of this technique. Furthermore, we have investigated the basic tissue clearing process in detail and have highlighted some areas for targeted improvement of technologies essential for the emerging subject of three-dimensional histology.
Date Issued
2016-06-30
Date Acceptance
2016-06-22
Citation
PLOS One, 2016, 11 (6)
ISSN
1932-6203
Publisher
Public Library of Science
Journal / Book Title
PLOS One
Volume
11
Issue
6
Copyright Statement
© 2016 Lai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Sponsor
Parkinson's UK
Parkinson's UK
Grant Number
K-1403
K-1403
Subjects
General Science & Technology
MD Multidisciplinary
Publication Status
Published
Article Number
e0158628