Localized growth factor delivery from microparticles modulates osteogenic and chondrogenic gene expression in a growth factor-dependent manner in an ex vivo chick embryonic bone model
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Growth factors play a crucial role in regulating various cellular functions, including proliferation and differentiation. Consequently, the biomaterial-based delivery of exogenous growth factors presents a promising strategy in regenerative medicine to manage the healing process and restore tissue function. For effective therapeutic applications, it is essential that these active compounds are precisely targeted to the site of regeneration, with release kinetics that align with the gradual pace of tissue growth. We have developed an ex vivo model utilizing a developing embryonic chick bone, and using PLGA-based microparticles as controlled-release systems, allowing for the investigation of the spatiotemporal effects of growth factor delivery on cell differentiation and tissue formation. Our findings demonstrate that BMP2 and FGF2 can significantly alter cell morphology and zonally pattern collagen deposition within the model, but only when the growth factor presentation rate is carefully regulated. Furthermore, the growth factor-dependent responses observed underscore the potential of this model to explore interactions between cells and the growth factors released from biomaterials in an approach which can be applied to bone tissue engineering.
Date Issued
2025-09-15
Date Acceptance
2025-08-12
Citation
Acta Biomaterialia, 2025, 204, pp.558-567
ISSN
1742-7061
Publisher
Elsevier BV
Start Page
558
End Page
567
Journal / Book Title
Acta Biomaterialia
Volume
204
Copyright Statement
© 2025 The Authors. Published by Elsevier Inc. on behalf of Acta Materialia Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40816552
PII: S1742-7061(25)00610-5
Subjects
BMP2
Chick embryonic bone
Controlled release
FGF2
Microparticles
PLGA
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2025-08-13