A first step towards practical single cell proteomics: a microfluidic antibody
capture chip with TIRF detection
capture chip with TIRF detection
File(s)
Author(s)
Type
Journal Article
Abstract
We have developed a generic platform to undertake the analysis of protein copy number from singlecells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
Date Issued
2011-02-23
Citation
Lab Chip, 2011, 11, pp.1256-1261
ISSN
1473-0197
Publisher
ROYAL SOC CHEMISTRY
Start Page
1256
End Page
1261
Journal / Book Title
Lab Chip
Volume
11
Issue
7
Copyright Statement
© The Royal Society of Chemistry 2011
Description
20/09/12 meb, Author version attached, OK to publish.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000288455100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Notes
PubMed ID: 21347466
Publication Status
Published