Unsymmetric or hybrid detergents for membrane protein structural study
File(s)
Accepted version
Author(s)
Moon, Youngsun
Lee, Yewon
Lee, Ho Jin
Byrne, Bernadette
Chae, Pil Seok
Type
Journal Article
Abstract
Detergent micelles are widely used as a membrane-mimetic system for membrane protein extraction, isolation and structural study. Many recently developed detergents feature multiple tail and head groups, with architectures that are symmetric (i.e. the same alkyl chain) and non-hybrid (single head group type). Further diversification has involved incorporating structural differences in the tail groups (unsymmetric), head groups (hybrid), or both head and tail groups (unsymmetric hybrid). In this mini-review, we introduce these novel detergents, focusing on the relationships between their structural features, physical properties and performance in membrane protein applications. The detergent design strategy utilizing unsymmetric/hybrid structures expands the detergent repertoire and the detergent structure–property-efficacy relationships presented offer valuable design guidelines, collectively advancing membrane protein research.
Date Issued
2025-08-01
Date Acceptance
2025-06-01
Citation
Current Opinion in Structural Biology, 2025, 93
ISSN
0959-440X
Publisher
Elsevier BV
Start Page
103074
End Page
103074
Journal / Book Title
Current Opinion in Structural Biology
Volume
93
Copyright Statement
© 2025 Elsevier Ltd. All rights are reserved. This is the author’s accepted manuscript made available under a CC-BY licence in accordance with Imperial’s Research Publications Open Access policy (www.imperial.ac.uk/oa-policy)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40460545
PII: S0959-440X(25)00092-2
Subjects
Detergents
Membrane Proteins
Micelles
Protein Conformation
Humans
Publication Status
Published
Coverage Spatial
England
Article Number
103074
Date Publish Online
2025-06-02