Functional microvascularization of human myocardium in vitro
File(s)
Author(s)
Type
Journal Article
Abstract
In this study, we report static and perfused models of human myocardial-microvascular interaction. In static culture, we observe distinct regulation of electrophysiology of human induced pluripotent stem cell derived-cardiomyocytes (hiPSC-CMs) in co-culture with human cardiac microvascular endothelial cells (hCMVECs) and human left ventricular fibroblasts (hLVFBs), including modification of beating rate, action potential, calcium handling, and pro-arrhythmic substrate. Within a heart-on-a-chip model, we subject this three-dimensional (3D) co-culture to microfluidic perfusion and vasculogenic growth factors to induce spontaneous assembly of perfusable myocardial microvasculature. Live imaging of red blood cells within myocardial microvasculature reveals pulsatile flow generated by beating hiPSC-CMs. This study therefore demonstrates a functionally vascularized in vitro model of human myocardium with widespread potential applications in basic and translational research.
Date Issued
2022-09-19
Date Acceptance
2022-08-11
Citation
Cell Reports: Methods, 2022, 2 (9), pp.1-16
ISSN
2667-2375
Publisher
Elsevier
Start Page
1
End Page
16
Journal / Book Title
Cell Reports: Methods
Volume
2
Issue
9
Copyright Statement
© 2022 Imperial College London.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000907954800003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
ANGIOGENESIS
Biochemical Research Methods
Biochemistry & Molecular Biology
BLOOD-FLOW
CARDIAC MICROTISSUES
CARDIOMYOCYTES
Cell Biology
ENDOTHELIUM
FIBROBLASTS
GAP-JUNCTIONS
HEART
Life Sciences & Biomedicine
MODULATION
Science & Technology
STEM-CELLS
Publication Status
Published
Date Publish Online
2022-08-29