Externally imposed electric field enhances plant root tip regeneration
File(s)
Accepted version
Published version
Author(s)
Kral, N
Hanna Ougolnikova, A
Sena, G
Type
Journal Article
Abstract
In plants, shoot and root regeneration can be induced in the distinctive conditions of
tissue culture (in vitro), but is also observed in intact individuals (in planta) recovering
from tissue damage. Roots, for example, can regenerate their fully excised meristems in
planta, even in mutants with impaired apical stem cell niches. Unfortunately, to date a
comprehensive understanding of regeneration in plants is still missing.
Here, we provide evidence that an imposed electric field can perturb apical root
regeneration in Arabidopsis. Crucially, we explored both spatial and temporal
competences of the stump to respond to electrical stimulation, respectively by varying
the position of the cut and the time interval between excision and stimulation.
Our data indicate that a brief pulse of an electric field parallel to the root is sufficient to
increase by up to two-fold the probability of its regeneration, and to perturb the local
distribution of the hormone auxin, as well as cell division regulation. Remarkably, the
orientation of the root towards the anode or the cathode is shown to play a role.
tissue culture (in vitro), but is also observed in intact individuals (in planta) recovering
from tissue damage. Roots, for example, can regenerate their fully excised meristems in
planta, even in mutants with impaired apical stem cell niches. Unfortunately, to date a
comprehensive understanding of regeneration in plants is still missing.
Here, we provide evidence that an imposed electric field can perturb apical root
regeneration in Arabidopsis. Crucially, we explored both spatial and temporal
competences of the stump to respond to electrical stimulation, respectively by varying
the position of the cut and the time interval between excision and stimulation.
Our data indicate that a brief pulse of an electric field parallel to the root is sufficient to
increase by up to two-fold the probability of its regeneration, and to perturb the local
distribution of the hormone auxin, as well as cell division regulation. Remarkably, the
orientation of the root towards the anode or the cathode is shown to play a role.
Date Issued
2016-08-20
Date Acceptance
2016-06-29
Citation
Regeneration, 2016, 3 (3), pp.156-167
ISSN
2052-4412
Publisher
Wiley
Start Page
156
End Page
167
Journal / Book Title
Regeneration
Volume
3
Issue
3
Copyright Statement
© 2016 The Authors.Regeneration published by John Wiley & Sons Ltd. This is an open access article under theterms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,provided the original work is properly cited.
License URL
Sponsor
BBSRC
Grant Number
BB/J014575
Subjects
Arabidopsis
Plant regeneration
plant root
Publication Status
Published