Synthetic biology of Fusarium for the sustainable production of valuable bioproducts
File(s)
Published version
Author(s)
Gnaim, Rima
Ledesma-Amaro, Rodrigo
Type
Journal Article
Abstract
Synthetic biology offers transformative opportunities to optimise Fusarium species as efficient platforms for the sustainable production of diverse bioproducts. Advanced engineering techniques, including CRISPR/Cas9, RNA interference and synthetic promoters, have enhanced the manipulation of metabolic pathways, enabling higher yields of industrially relevant compounds. Recent insights from next-generation sequencing and omics technologies have significantly expanded our understanding of Fusarium's metabolic networks, leading to more precise strain engineering. Despite these advances, challenges such as metabolic bottlenecks, regulatory complexities and strain stability remain significant barriers to industrial-scale applications. The development of efficient genetic tools, together with the expansion of our knowledge of Fusarium physiology and genetics thanks to systems biology approaches, holds promise to unlock Fusarium's full potential as a sustainable cell factory. This review focuses on the genetic and metabolic tools available to enhance Fusarium's capacity to produce biofuels, pharmaceuticals, enzymes and other valuable compounds. It also highlights key innovations and discusses future directions for leveraging Fusarium as an environmentally friendly bioproduction system.
Date Issued
2025-07-01
Date Acceptance
2025-04-08
Citation
Biotechnology Advances, 2025, 81
ISSN
0734-9750
Publisher
Elsevier BV
Journal / Book Title
Biotechnology Advances
Volume
81
Copyright Statement
© 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40222460
PII: S0734-9750(25)00065-5
Subjects
Alternative protein
Biomass fermentation
Bioproduct
Filamentous fungi
Fusarium
Metabolic engineering
Synthetic biology
Fusarium
Synthetic Biology
Metabolic Engineering
Metabolic Networks and Pathways
Biofuels
CRISPR-Cas Systems
Publication Status
Published
Coverage Spatial
England
Article Number
108579
Date Publish Online
2025-04-11