A biochemical comparison of the lung, colonic, brain, renal, and ovarian cancer cell lines using 1H-NMR spectroscopy
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Cancer cell lines are very often used for cancer research. However, continuous genetic instability-induced heterogeneity of cell lines can hinder the reproducibility of cancer research. Molecular profiling approaches including transcriptomics, chromatin modification profiling and proteomics are used to evaluate the phenotypic characteristics of cell lines. However, these do not reflect the metabolic function at the molecular level. Metabolic phenotyping is a powerful tool to profile the biochemical composition of cell lines. In this study, 1H-NMR spectroscopy-based metabolic phenotyping was used to detect metabolic differences among 5 cancer cell lines, namely, lung (A549), colonic (Caco2), brain (H4), renal (RCC), and ovarian (SKOV3) cancer cells. The concentrations of choline, creatine, lactate, alanine, fumarate and succinate varied remarkably among different cell types.The significantly higher intracellular concentrations of glutathione, myo-inositol, and phosphocholine were found in the SKOV3 cell line relative to other cell lines. Glutamate is higher in both SKOV3 and RCC cells compared to other cell lines. For cell culture media analysis, isopropanol was found to be the highest in RCC media, followed by A549 and SKOV3 media, while acetone was the highest in A549, followed by RCC and SKOV3. These results demonstrated that 1H-NMR-based metabolic phenotyping approach allows us to characterize specific metabolic signatures of cancer cell lines and provides phenotypical information of cellular metabolism.
Date Issued
2020-04-23
Date Acceptance
2020-04-08
Citation
Bioscience Reports, 2020, 40 (4)
ISSN
0144-8463
Publisher
Portland Press Ltd.
Journal / Book Title
Bioscience Reports
Volume
40
Issue
4
Copyright Statement
© 2020 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution
License 4.0 (CC BY)
License 4.0 (CC BY)
Sponsor
Medical Research Council (MRC)
Commission of the European Communities
Identifier
https://portlandpress.com/bioscirep/article/doi/10.1042/BSR20194027/222600/A-Biochemical-Comparison-of-the-Lung-Colonic-Brain
Grant Number
MR/P002536/1
715662
Subjects
NMR spectroscopy
colonic cancer
glioma
lung cancer
metabolomics
renal cancer
0601 Biochemistry and Cell Biology
Biochemistry & Molecular Biology
Publication Status
Published
Date Publish Online
2020-04-08