Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates
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Supporting information
Published version
Author(s)
Type
Journal Article
Abstract
The throwaway culture related to the single-use materials such as polyethylene terephthalate (PET) has created a major environmental concern. Recycling of PET waste into biodegradable plastic polyhydroxyalkanoate (PHA) creates an opportunity to improve resource efficiency and contribute to a circular economy. We sequenced the genome of Pseudomonas umsongensis GO16 previously shown to convert PET-derived terephthalic acid (TA) into PHA and performed an in-depth genome analysis. GO16 can degrade a range of aromatic substrates in addition to TA, due to the presence of a catabolic plasmid pENK22. The genetic complement required for the degradation of TA via protocatechuate was identified and its functionality was confirmed by transferring the tph operon into Pseudomonas putida KT2440, which is unable to utilise TA naturally. We also identified the genes involved in ethylene glycol (EG) metabolism, the second PET monomer, and validated the capacity of GO16 to use EG as a sole source of carbon and energy. Moreover, GO16 possesses genes for the synthesis of both medium and short chain length PHA and we have demonstrated the capacity of the strain to convert mixed TA and EG into PHA. The metabolic versatility of GO16 highlights the potential of this organism for biotransformations using PET waste as a feedstock.
Date Issued
2021-11-01
Date Acceptance
2020-11-03
Citation
Microbial Biotechnology, 2021, 14 (6), pp.2463-2480
ISSN
1751-7907
Publisher
Wiley
Start Page
2463
End Page
2480
Journal / Book Title
Microbial Biotechnology
Volume
14
Issue
6
Copyright Statement
© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
Biotechnology and Biological Sciences Research Council
Grant Number
BB/T011289/1
Subjects
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Microbiology
AROMATIC CATABOLIC PATHWAYS
PUTIDA KT2440
ENTNER-DOUDOROFF
PLASTIC WASTE
DEGRADATION
ALIGNMENT
BACTERIUM
SEQUENCE
TEREPHTHALATE
PHYLOGENY
0605 Microbiology
Publication Status
Published
Date Publish Online
2021-01-06