Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Analyzing host-microbe interactions is essential for understanding how microbiota changes disrupt host homeostasis. Here, we present a protocol for predicting host-microbe protein-protein interactions and their downstream effects using MicrobioLink. We describe steps for setting up the environment, installing software, and preparing human transcriptomic and bacterial proteomic data. The protocol outlines procedures for predicting protein-protein interactions through domain-motif interactions, integrating multi-omic datasets to map downstream effects, performing network analyses to identify key regulatory pathways, and visualizing multi-layered networks for systems-level data interpretation.
For complete details on the use and execution of this protocol, please refer to Gul et al.¹ and Poletti et al.²
For complete details on the use and execution of this protocol, please refer to Gul et al.¹ and Poletti et al.²
Date Issued
2025-03-21
Date Acceptance
2025-01-01
Citation
STAR Protocols, 2025, 6 (1)
ISSN
2666-1667
Publisher
Elsevier
Journal / Book Title
STAR Protocols
Volume
6
Issue
1
Copyright Statement
© 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/39826120
PII: S2666-1667(24)00735-4
Subjects
Biochemical Research Methods
Biochemistry & Molecular Biology
Life Sciences & Biomedicine
Science & Technology
Publication Status
Published
Coverage Spatial
United States
Article Number
103570
Date Publish Online
2025-01-17