Transcriptome and cell physiological analyses in different rice cultivars provide new insights into adaptive and salinity stress responses
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H2O2 in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H2O2 production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H2O2, a reduction in the expression of these genes was detected. Our results indicate that quick H2O2 signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants.
Date Issued
2018-03-05
Date Acceptance
2018-02-02
Citation
Frontiers in Plant Science, 2018, 9
ISSN
1664-462X
Publisher
Frontiers Media
Journal / Book Title
Frontiers in Plant Science
Volume
9
Copyright Statement
© 2018 Formentin, Sudiro, Perin, Riccadonna, Barizza, Baldoni, Lavezzo, Stevanato, Sacchi, Fontana, Toppo, Morosinotto, Zottini and Lo Schiavo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Subjects
Science & Technology
Life Sciences & Biomedicine
Plant Sciences
Oryza sativa (rice)
salt stress
RNA sequencing
ion transporters
H2O2
salt tolerance mechanisms
GENE-EXPRESSION DATA
RNA SEQUENCING DATA
ORYZA-SATIVA L.
SALT STRESS
OSMOTIC-STRESS
NADPH OXIDASE
BIOCONDUCTOR PACKAGE
FUNCTION PREDICTION
NA+/K+ HOMEOSTASIS
DROUGHT TOLERANCE
Publication Status
Published
Article Number
ARTN 204
Date Publish Online
2018-03-05