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Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections
File(s)
emi12856.pdf (1.69 MB)
Accepted version
Author(s)
Aragon, IM
Pérez-Mendoza, D
Moscoso, JA
Faure, E
Guery, B
Type
Journal Article
Abstract
The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosa PAK led to increased exopolysaccharide production and upregulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. savastanoi pv. savastanoi NCPPB 3335 and P. aeruginosa PAK reduced their virulence in olive plants and in a mouse acute lung injury model respectively. Our results show that diguanylate cyclase DgcP is a conserved Pseudomonas protein with a role in virulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulating plant and human Pseudomonas spp. infections.
Date Issued
2015-04-28
Date Acceptance
2015-03-06
Citation
Environmental Microbiology, 2015, 17 (11), pp.4332-4351
URI
DOI
ISSN
1462-2920
Publisher
Wiley
Start Page
4332
End Page
4351
Journal / Book Title
Environmental Microbiology
Volume
17
Issue
11
Copyright Statement
© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Aragon, I. M., Pérez-Mendoza, D., Moscoso, J. A., Faure, E., Guery, B., Gallegos, M.-T., Filloux, A. and Ramos, C. (2015), Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environmental Microbiology, which has been published in final form at https://dx.doi.org/10.1111/1462-2920.12856. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
License URL
Publication Status
Published